Abstract
Pathological vascular endothelial damage caused by hypoxia is the basis of many vascular-related diseases. However, the role of circular RNA in hypoxic vascular injury is still poorly understood. Here, we found that hypoxia induced AFF1 circular RNA (circAFF1) can activate the SAV1/YAP1 and lead to the dysfunction of vascular endothelial cells. In HUV-EC-C and HBEC-5i cells, circAFF1 was upregulated under CoCl2 induced hypoxic conditions. The abnormal expression of circAFF1 inhibited the proliferation, tube formation, migration of vascular endothelial cells. The effect of circAFF1 is achieved by the adsorption of miR-516b to release SAV1, which in turn causes the phosphorylation of YAP1. Moreover, we found that the upregulation of circAFF1 in 235 Patients with subarachnoid hemorrhage. Taken together, we clarify the role of circAFF1/miR-516b/SAV1/YAP1 axis in vascular endothelial dysfunction and its potential early diagnostic value of disease caused by hypoxia injury in blood vessels.
Highlights
Ischemia occurs when local tissues cannot meet their energy needs, and hypoxia is an important component of ischemic injury (Eltzschig et al, 2014)
We searched circAFF1 on the circBase1 and found that circAFF1 was derived from exons 3 and 4 of the AFF1 gene (Figure 1C)
These results indicated that circAFF1 was upregulated in the endothelial cells under hypoxic conditions and was predominantly localized in the cytoplasm
Summary
Ischemia occurs when local tissues cannot meet their energy needs, and hypoxia is an important component of ischemic injury (Eltzschig et al, 2014). Hypoxia plays an important role in protective and pathological vascular adaptation. Hypoxia causes damage to vascular endothelial cells, and endothelial dysfunction disrupts blood flow (Mao et al, 2013; Giannarelli et al, 2014; Zhao et al, 2015). The occurrence and development of vascular endothelial injury is an important. CircAFF1 Aggravates Vascular Dysfunction cause of many diseases, such as cardiovascular and cerebrovascular diseases, diabetes and tumors (Shan et al, 2017; Qureshi-Baig et al, 2019). Whether vascular dysfunction will accelerate the development of these diseases and how to achieve early diagnosis and prevention has received little attention
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