Abstract
A link between the circadian rhythm and the function of Cl(-)-permeable gamma-aminobutyric acid (GABA) type A (GABA(A)) receptors on suprachiasmatic nucleus (SCN) neurons was studied by measuring intracellular activity of Cl(-) (aCl) at different times during a circadian cycle in SCN neurons acutely dissociated from rat brains. To measure aCl, the voltage-clamp mode of the gramicidin-perforated patch-clamp technique was used, and reversal potential of GABA-induced currents (E(GABA)) was converted to aCl. Measured aCl was significantly higher at around noon (20.1 +/- 1.4 mM) than at three other time zones of a circadian cycle (means ranging from 11.6 to 14.3 mM). Chord conductance of GABA-induced currents showed no circadian changes, indicating a lack of circadian changes in the number or single-channel conductance of GABA(A) receptors. These results suggest that aCl participates in modulating GABA(A) receptor functions on SCN neurons during the circadian rhythm.
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