Circadian control of singing in crickets: Two different pacemakers for early-evening and before-dawn activity

Abstract

(1) Under LD 12:12 and constant temperature, calling song patterns of the Australian Field Cricket Teleogryllus commodus were frequently divided into two more or less distinct activity components. The first began 1–3 h before the L D -transition and ended with the sudden lights-off, whereas the second was restricted mainly to the second half of the night. In subsequent continuous light (LL) the circadian activity pattern was usually a unimodal band. Its onset slope was always a continuation of the first onsets in the preceding LD-cycle. In those cases where shortly after the LD LL transition the rhythm split into two components, both corresponded to those in LD. (2) In “atypical” LD-aatterns only the second component appeared during the late night. In such cases, the onset slope of the subsequent free-running rhythm did not start at the preceding LD-entrained onsets, but was advanced by several hours. (3) In free-running rhythms the mean value of the onset slopes ( τ o = 25.1 ± 0.07 h ) did not differ significantly from that of the end slopes ( τ e = 25.0 ± 0.07 h ). However, in individual activity patterns, simultaneous differences in onset and end slopes of up to 0.7 h were found. τ o- and τ e-values recorded directly after the LD LL -transition did not correlate with the preceding phase angle difference between the entrained activity and the zeitgeber. (4) Under constant conditions after-effects such as spontaneous period changes and phase shifts in the slopes of activity onset and/or end occurred mainly between five and 20 days after the LD LL -transition . (5) Period changes and phase delays in the onset and end slopes were also found when crickets were exposed to low temperature pulses (2 ± 2°C, 2h duration). Following the cold treatment both period changes and phase shifts in the onset slope frequently differed from those in the end slope. (6) The results are consistent with a concept of two activity components controlled by weakly coupled circadian pacemakers. This is clearly evident in split rhythms but is also true for unimodal patterns. Normally the two components partly overlap and their existence is concealed in the undivided activity band. They are only revealed in unimodal patterns if individual oscillatory properties such as different period lengths or phase shifts appear in the onset or the end slope.