Abstract

A culture of house crickets Acheta domisticus (L) was maintained under light-dark, 12:12 photoperiod. The activities of cholinesterase (ChE) and carboxylesterase (CarE) of rhythmic adults were assayed at different times of the day. Both enzymes showed a daily rhythmic pattern of activity. ChE had its highest activity level at 1200 hr, while CarE showed two peaks of activity, at 1200 and 2400 hr. The lowest level of activity for both enzymes was recorded shortly after the onset of darkness. The effect of these rhythmic fluctuations on the inhibitory action of phorate was studied. Insects were topically treated with a sublethal dose of phorate. Phorate application took place at different intervals to coincide with different magnitudes of enzymes activity. ChE inhibition did not differ significantly in relation to the time of phorate application, however, CarE inhibition was much higher when phorate was applied at the beginning of the dark phase. Inhibited ChE showed a rapid recovery only in insects treated in the light phase.

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