Circadian and ultradian rhythms of body temperature and peripheral concentrations of insulin and nitrogen in lactating dairy cows

Abstract

To investigate possible circadian and ultradian periodicities for peripheral insulin and urea in lactating dairy cows, integrated 15-min blood samples were taken sequentially over 48 hr from six cows. In addition, radiotelemetry measurements of body temperature were averaged over the same 15-min periods. Cows were housed in an environmental chamber at 19°C with lights on 0700 to 2300 hr; fed daily at 0900 hr; and milked at 0800 and 2000 hr. For five of the six cows, body temperature showed a circadian rhythm peaking at 2323 hr with an amplitude of 0.34°C. For the sixth cow, body temperature was 180° out-of-phase, peaking at 1230 hr with an amplitude of 0.12°C. Circadian rhythms for insulin and urea were consistent for all six cows peaking at 1743 hr with an amplitude of 0.74 ng/ml for insulin and at 1034 hr with an amplitude of 3.83 mM for urea. Body temperature and insulin also displayed episodic increases that often exceeded the amplitudes of circadian rhythms. For body temperature, a broad increase in spectral power was seen for periods between 100 and 175 min; time intervals between peaks averaged around 100 min. For insulin, power spectra for individual cows universally indicated rhythms with periods of approximately 45 and 80 min; time intervals between peaks averaged approximately 65 min. For urea, almost all spectral energy was confined to the 24-hr rhythm, although there was evidence of a low-amplitude, 60-min rhythm. In conclusion, when animals are acclimated to a rigidly controlled environment and frequent blood sampling is accomplished with minimal intervention, it is possible to detect rhythms inherent in the regulation of metabolic variables.