Abstract
BackgroundNon-small-cell lung cancer (NSCLC) is one of the common cancers in the world. Circular RNA 0072083 (circ_0072083, circZFR) has been reported to be associated with the progression of NSCLC. In this study, we intended to explore the role and the potential mechanism of circ_0072083 in NSCLC.MethodsQuantitative real time polymerase chain reaction (qRT-PCR) was performed to detect the expression of circ_0072083, its matching linear RNA (zinc finger RNA binding protein (ZFR)) and microRNA-545-3p (miR-545-3p) in NSCLC cells. The ability of colony formation in NSCLC cells was detected by colony formation assay. The apoptosis and cell cycle were measured by flow cytometry. The metastasis was determined by transwell migration and invasion assays. The protein expression of E-cadherin, N-cadherin, Vimentin and Cbl proto-oncogene like 1 (CBLL1) was examined by western blot assay. The interaction between miR-545-3p and circ_0072083 or CBLL1 was predicted by starBase or Targetscan software. Dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay were applied to validate these interactions. Nude mice bearing tumors were used to confirm the role of circ_0072083 and cisplatin (DDP) in vivo.ResultsThe level of circ_0072083 was higher in NSCLC tissues and cells relative to that in adjacent non-tumor tissues and normal lung cells. The transfection of si-circ_0072083 inhibited colony formation, cell cycle and metastasis while promoted the apoptosis of NSCLC cells stimulated by DDP. MiR-545-3p was a direct functional target of circ_0072083 in NSCLC cells. CBLL1 could bind to miR-545-3p in NSCLC cells. Circ_0072083 promoted the progression of NSCLC induced by DDP through sponging miR-545-3p and enhancing the enrichment of CBLL1 in vivo and in vitro.ConclusionCirc_0072083 depletion contributed to DDP-triggered inhibition of NSCLC tumor through miR-545-3p/CBLL1 axis.
Highlights
Non-small-cell lung cancer (NSCLC) is one of the common cancers in the world
We investigated whether miR545-3p exerted its function through Cbl proto-oncogene like 1 (CBLL1) in NSCLC
Compared with matching linear messenger RNA, circ_0072083 was more stable owing to its closed loop structure (Fig. 1e, f )
Summary
Non-small-cell lung cancer (NSCLC) is one of the common cancers in the world. We intended to explore the role and the potential mechanism of circ_0072083 in NSCLC. Lung cancer is the leading cause of the cancer-associated mortality globally [1, 2]. There were 2093,876 new cases and 1,761,007 deaths of lung cancer globally in 2018, accounting for 11.6% and 18.4% of all cancers, respectively [2]. Among the two types of lung cancer, NSCLC accounts for 85% [3]. The therapeutic methods for NSCLC patients include surgery resection, chemotherapy, radiotherapy, targeted therapy and immunotherapy [4]. Uncovering the resistance mechanism of NSCLC cells is urgent to avoid the metastasis or neoplasm recurrence
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
