Abstract
BackgroundPleckstrin homology-like domain family A member 1 (PHLDA1) is a tumor suppressor gene in gastric cancer, but its role regulated by circular RNAs (circRNAs) is not known. CircRNAs are important regulators in cancer growth and progression, however, the molecular roles of circRNAs in gastric cancer are rarely known. The study was aimed to investigate the role of circRNAs in regulating PHLDA1 expression in gastric cancer.ResultsThe circRNA expression profile in the gastric cancer tissues by circRNA microarray showed that hsa_circ_0027599 (circ_0027599) was significantly down-regulated in gastric cancer patients and cells when comparing with the controls. Circ_0027599 overexpression suppressed gastric cancer cell proliferation and metastasis. By using bioinformatics tools and luciferase reporter assays, circ_0027599 was verified as a sponge of miR-101-3p.1 (miR-101) and suppressed cancer cell survival and metastasis. It was also verified that PHLDA1 was regulated by circ_0027599 in gastric cancer cells.ConclusionsThe study uncovered that PHLDA1 was regulated by circ_0027599/miR-101, which suppressed gastric cancer survival and metastasis in gastric cancer.
Highlights
Pleckstrin homology-like domain family A member 1 (PHLDA1) is a tumor suppressor gene in gastric cancer, but its role regulated by circular RNAs is not known
PHLDA1 decreases gastric cancer cell survival and metastasis To investigate the role of PHLDA1 on gastric cancer cell survival ability, firstly, SGC-7901, MGC-803, HGC27, MKN-45, MKN-28 gastric cancer cells and human stomach epithelial cells (HPSEC) were used to examine PHLDA1 expression, it was found that PHLDA1 expression was lower in MKN-28 and HGC-27 cell lines than the other cell lines (Fig. 1a)
MKN28 and HGC-27 cells were transfected with PHLDA1 and cell proliferation was measured by CCK8 method
Summary
Pleckstrin homology-like domain family A member 1 (PHLDA1) is a tumor suppressor gene in gastric cancer, but its role regulated by circular RNAs (circRNAs) is not known. The study was aimed to investigate the role of circRNAs in regulating PHLDA1 expression in gastric cancer. It is pivotal to identify or find new biomarkers and therapeutic targets for improving gastric cancer prognosis. Better elucidating the mechanisms of gastric tumorigenesis and aggressiveness is important for improving the therapeutic efficiency of gastric cancer [2]. Pleckstrin homology-like domain family A member 1 (PHLDA1) protein is encoded by the PHLDA1 gene [3]. This gene encodes an evolutionarily conserved proline-histidine rich nuclear protein [3]. PHLDA1 could function as an oncogene or a tumor suppressor gene in
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