Circ_0008792 Prevents Against the Deterioration of Thyroid Cancer Through Regulating IGFBP5

Abstract

To detect the expression of circ_0008792 in thyroid cancer (TC) tissues and cell lines, and to explore the regulatory effect of circ_0008792 on TC progression. Relative levels of circ_0008792 and IGFBP5 in TC and paracancerous tissues were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The influences of circ_0008792 and IGFBP5 on pathology and prognosis of TC patients were analyzed. After intervening circ_0008792 level by transfection, changes in proliferative and migratory abilities of BHP5-16 and TPC cells were analyzed by cell counting kit-8 (CCK-8) and transwell assay, respectively. The interaction between circ_0008792 and IGFBP5 was explored by dual-luciferase reporter assay. In vivo xenograft model of TC was constructed in nude mice. Co-regulation of circ_0008792 and IGFBP5 on in vitro and In vivo TC progression was respectively analyzed. circ_0008792 was lowly expressed in TC tissues. TC patients overexpressing circ_0008792 had a relatively well outcome, manifesting as earlier tumor staging, lower rate of lymphatic metastasis and higher overall survival in comparison to whom expressing low level of circ_0008792. Overexpression of circ_0008792 in TC cells attenuated proliferative and migratory abilities, and knockdown of circ_0008792 yielded the opposite results. IGFBP5 was the target gene binding circ_0008792, which was negatively correlated to circ_0008792 in TC tissues. circ_0008792 overexpression attenuated proliferative and migratory abilities in TC cells, and slowed down In vivo growth of TC by targeting IGFBP5. circ_0008792 is lowly expressed in TC, and its level is correlated to tumor staging, lymphatic metastasis rate and overall survival of TC. It inhibits proliferative and migratory potentials in TC cells by regulating IGFBP5.