Circ-0002814 participates in proliferation and migration through miR-210 and FUS/VEGF pathway of preeclampsia.

Abstract

Circular RNA plays a critical role in cell proliferation, differentiation, and autophagy. However, the role and mechanism of circRNA in preeclampsia is yet to be clarified. The present study investigated the function and mechanism of circ-0002814 in preeclampsia. The expression level of circ-0002814, microRNA 210 (miR210), Notch receptor 1 (Notch1), or cytoplasmic polyadenylation element-binding protein 2 (CPEB2) was investigated by Reverse transcription Real-time Quantitative PCR (RT-qPCR). The level of Notch1, CPEB2, and FUS (FUS RNA-binding protein) proteins was detected by western blot. The localization of circ-0002814 and miR-210 was determined by FISH assay. Cell proliferation and invasion were detected by EdU and transwell assays, respectively. The interaction between circ-0002814 and FUS protein was detected by RNA pulldown assay, while that between circ-0002814 and miR-210 was detected by dual-luciferase reporter assay. The expression of circ-0002814 was decreased in the placenta and plasma of patients with preeclampsia. The ectopic expression of circ-0002814 promoted the invasion and proliferation of HTR8 cells compared to the control group (p < 0.05). Silencing circ-0002814 suppressed the invasion and proliferation of JEG3 cells compared to the control group (p < 0.05). circ_0002814-regulated Notch1 and CPEB2 by interaction with miR-210 and also regulated FUS/VEGF axis in HTR8 and JEG3 cells. A low expression of circ-0002814 was detected in the plasma and placental tissue in preeclampsia patients. circ-0002814 participated in the proliferation and invasion of HTR8 and JEG3 cells. Thus, circ-0002814 may be considered as a potential diagnostic marker and therapeutic target for preeclampsia.