Abstract
Background: Increasing circular RNAs (circRNAs) are reported to participate in cancer progression. Nonetheless, the role of circRNAs in nasopharyngeal carcinoma (NPC) has not been fully clarified. This work is aimed to probe the role of circ_0000215 in NPC.Methods: Circ_0000215 expression in NPC tissues and cell lines was examined by quantitative real-time polymerase chain reaction (qRT-PCR). Cell counting kit-8 (CCK-8) assay, 5-bromo-2′-deoxyuridine (BrdU) assay, scratch healing assay and Transwell experiment were executed to investigate the regulatory function of circ_0000215 on the proliferation, migration and invasion of NPC cells. RNA immunoprecipitation (RIP), pull-down and dual-luciferase reporter experiments were utilized to determine the binding relationship between circ_0000215 and miR-512-5p, and between miR-512-5p and phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1) 3′UTR. The effects of circ_0000215 on NPC growth and metastasis in vivo were examined with nude mice model. Western blot was applied to detect the regulatory effects of circ_0000215 and miR-512-5p on PIK3R1 expression.Results: Circ_0000215 was overexpressed in NPC tissues and cell lines. The functional experiments confirmed that knockdown of circ_0000215 impeded the growth and metastasis of NPC cells in vitro and in vivo. Additionally, circ_0000215 could also work as a molecular sponge to repress miR-512-5p expression. PIK3R1 was validated as a target gene of miR-512-5p, and circ_0000215 could increase the expression level of PIK3R1 in NPC cells via suppressing miR-512-5p.Conclusion: Circ_0000215 is overexpressed in NPC and exerts oncogenic effects in NPC through regulating miR-512-5p/PIK3R1 axis.
Highlights
Nasopharyngeal carcinoma (NPC) is a malignant epithelial neoplasm of the nasopharynx, and EBV infection is a known risk factor associated with NPC tumorigenesis and development (Global Cancer Observatory, 2018; Chen et al, 2019)
An increasing amount of evidence suggests that circRNAs are implicated in tumorigenesis and cancer progression by modulating important biological processes such as cell growth, migration, differentiation, drug resistance, metabolism, and so on (Hua et al, 2019; Kristensen et al, 2019; Yu et al, 2019)
We report that hsa_circ_0000215 [a circRNA derived from intron 2 and exons 3 of the calcium/calmodulin dependent protein kinase ID (CAMK1D), circ_0000215], was up-regulated in NPC tissue
Summary
Nasopharyngeal carcinoma (NPC) is a malignant epithelial neoplasm of the nasopharynx, and EBV infection is a known risk factor associated with NPC tumorigenesis and development (Global Cancer Observatory, 2018; Chen et al, 2019). An increasing amount of evidence suggests that circRNAs are implicated in tumorigenesis and cancer progression by modulating important biological processes such as cell growth, migration, differentiation, drug resistance, metabolism, and so on (Hua et al, 2019; Kristensen et al, 2019; Yu et al, 2019). Circ-ITCH restrains bladder cancer progression by sponging miR-17/miR-224 to modulate p21 and PTEN expressions (Yang et al, 2018). Increasing circular RNAs (circRNAs) are reported to participate in cancer progression. The role of circRNAs in nasopharyngeal carcinoma (NPC) has not been fully clarified. This work is aimed to probe the role of circ_0000215 in NPC
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