Abstract
Salmonella enterica serovar Kentucky (S. Kentucky) sequence type 198 has emerged as a global zoonotic pathogen. We explored Salmonella enterica serovar Kentucky ST198 samples from the broiler chicken supply chain and patients between 2010 and 2016. Here, we collected 180 S. Kentucky isolates from clinical cases and the poultry supply chain. We performed XbaI pulsed-field gel electrophoresis and multilocus sequence typing. We assessed mutations in the quinolone resistance-determining regions and screened for the presence of the Salmonella genomic island 1 (SGI1). We determined that 63 (35.0%) of the 180 isolates were S. Kentucky ST198. Chinese strains of S. Kentucky ST198 have a high transmission of ciprofloxacin resistance (38/63, 60.3%) and a high risk of multidrug resistance. The quinolone resistance of the S. Kentucky ST198 strain found in China may be due to mutations in its quinolone resistance-determining region. Our study firstly revealed that ciprofloxacin-resistant S. Kentucky ST198 strains can undergo cross-host transmission, thereby causing a serious foodborne public health problem in China.
Highlights
Salmonella enterica is a major global foodborne pathogen [1]
Microorganisms 2020, 8, 140 to the sequence of Salmonella genomic island 1 (SGI1)-Ks, SGI1-Ps, SGI1-Ps-Qs, SGI1-Qs synthetic primers (SGI1-Ks: forward: 5 -AAGGATTTCCTGACCCTG-3, reverse: 5 -ATGGATGTGGTGGCTGAAGG-3 ; SGI1-Ps: forward: 5 -CGGTTTTGAATAAGAAGGCA-3, reverse: 5 -CCAATGCTTAATCAGTGAGG-3 ; SGI1-Ps-Qs: forward: 5 -ATGAGTATTCAACATTTCCG-3, reverse: 5 -GTATTGTCGTCGGGATGATT-3 ; SGI1-Qs: forward: 5 -CGGTTTTGAATAAGAAGGCA-3, reverse: 5 -GTATTGTCGTCGGGATGATT-3 .), the total DNA of Salmonella was used as a template for Polymerase Chain Reaction (PCR) amplification, and the amplified products were detected by agarose gel electrophoresis [16]
Kentucky isolates were subtyped via pulsed-field gel electrophoresis (PFGE) [17] to determine their genetic relatedness according to the Pulse-Net protocol
Summary
Salmonella enterica is a major global foodborne pathogen [1]. In the United States, salmonellosis is the second leading cause of foodborne illnesses in humans [4], accounting for approximately 1 million cases, 19,336 hospitalizations, and 378 deaths each year. In China, approximately 70% to 80% of foodborne disease outbreaks are caused by Salmonella, and most of these disease outbreaks are associated with the ingestion of contaminated livestock and poultry products [5]. Salmonella enterica serotype Kentucky has rarely been associated with human illness. Enterica serovar Kentucky sequence type 198 (ST198) has emerged as a global human pathogen [8]. In China, there is currently no systematic study of the epidemiology and drug resistance of S. Kentucky ST198 isolates in China, which can support basic research, clinical practice, and public health risk forecasts for Salmonella
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
