Abstract
The kinetics of isomerization of D-glucose to D-fructose catalyzed by immobilized glucose isomerase from Bacillus coagulans were studied at 50, 60 and 70°C in a continuous stirred tank reactor. Initial substrate concentrations of 5 to 25% (W/V) were used with 0,043kg of immobilized enzyme per liter of reactor. From preliminary studies on the reactor, it was found that at a stirring speed of 2,25 r/s and a glucose residence time under 3 330s, diffusional interferences were negligible. Different rate models were tested, but only the Michaelis-Menten expression without inhibition adequately described the kinetic data. Non-linear estimations of Km and Vmax were determined at 50, 60 and 70°C respectively. The apparent activation energy obtained had a value of 46 kJ/mol.
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Schedule a callMore From: Canadian Institute of Food Science and Technology Journal/Journal de L'Institut Canadien de Science et Technologie Alimentaire
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