Cinnamon extract improves TNFa‐ induced overproduction of intestinal apolipoproteinB 48‐containing lipoproteins

Abstract

TNFa, stimulates the overproduction of intestinal apolipoproteins. We evaluated whether a water extract of cinnamon (Cinnulin PF®) improved the dyslipidemia induced by TNFa in Triton WR‐1339 treated hamsters, and whether Cinnulin PF® inhibits the TNFa‐induced over secretion of apoB‐48 in hamster enterocytes. In vivo, acute oral intake of Cinnulin PF® (50 mg per kg BW), inhibited the postprandial overproduction of apoB‐48 lipoproteins and serum triglycerides. Ex vivo 35S labeling studies, demonstrated that CE (10 and 20 ?g/mL) inhibited the over secretion of apoB‐48 induced by TNFa treated enterocytes. To determine the effects on the mRNA levels for the related genes of lipoprotein metabolism in enterocytes, fresh isolated primary enterocytes of hamsters were pre‐treated with TNFa(10 ng per mL for 30min), then treated with and without Cinnulin PF® (10ug/mL) for 0.5, 2h and 4h. Quantitative real‐time PCR assays demonstrated that Cinnulin PF® improved the mRNA expression of IR, IRS1, IRS2, PI3K and Akt1and decreased the mRNA expression of MTP, PTEN, IL1a and b, IL6 and TNFa in TNFa treated enterocytes. In summary, our results suggest that a water extract of cinnamon reverses TNFa‐induced overproduction of intestinal apoB‐48 via regulating the gene expression of components of the insulin signaling, lipoprotein and inflammatory pathways. (Funded by USDA/ARS and Integrity Nutraceuticals).