Cinnabarinic acid from Trametes coccinea fruiting bodies exhibits antibacterial activity through inhibiting the biofilm formation.

Merilin Kakoti,Madhumita Barooah,Samim Dullah,Robin Chandra Boro,Dibya Jyoti Hazarika

doi:10.1007/s00203-022-02782-4

Merilin Kakoti, Madhumita Barooah + Show 3 more

Open Access

https://doi.org/10.1007/s00203-022-02782-4

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Abstract

Wild mushrooms are rich sources of natural compounds with potent bioactive properties. Several important metabolites have been reported from mushrooms, which possess clinically important bioactive properties like antibacterial, anticancer, antidiabetic, and neuroprotective activity. In this study, we have evaluated the antimicrobial activity of Trametes coccinea fruiting body extracts against different bacterial isolates, viz., Bacillus subtilis, Bacillus cereus, and Escherichia coli. Fruiting bodies of three T. coccinea samples, of which two were collected from Santipur, Arunachal Pradesh and one collected from Jorhat, Assam, were used for extraction using methanol. The extracts showed significant antimicrobial activity against all the test bacteria. Minimum Inhibitory Concentration (MIC) of the extracts against Bacillus subtilis, Bacillus cereus, and Escherichia coli was recorded as 400µg/ml, 400µg/ml, and 300µg/ml, respectively. Furthermore, the bioactive compounds of the extract were separated and detected using Thin Layer Chromatography (TLC). Presence of cinnabarinic acid (CBA)-a potent antimicrobial compound- was detected in TLC, which was further confirmed through High Performance Liquid Chromatography (HPLC) and Electrospray Ionization-Mass Spectrometry (ESI-MS). Cinnabarinic acid was able to inhibit the formation of biofilms in Bacillus subtilis and B. cereus, suggesting that the compound can be beneficial in the management of biofilm-based antimicrobial resistance.

Highlights

Antibiotics have gained place among the most important medical interventions needed for the treatment of complex clinical causalities
We have evaluated the antimicrobial activity of Trametes coccinea fruiting body extracts against different bacterial isolates viz. Bacillus subtilis, Bacillus cereus and Escherichia coli
Presence of Cinnabarinic acid (CBA), a potent antimicrobial compound was detected in Thin Layer Chromatography (TLC), which was further confirmed through High Performance Liquid Chromatography (HPLC) and Electrospray Ionization-Mass Spectrometry (ESI-MS)

Summary

Introduction

Antibiotics have gained place among the most important medical interventions needed for the treatment of complex clinical causalities. Many of the antibiotics have failed to serve in persistent manner because of resistance mechanisms employed by several pathogenic bacteria. These mechanisms include enzymatic degradation or alteration of antibiotic molecules, mutational changes of the antibiotic target sites, decreased permeability of antibiotics inside the cells, efflux of antibiotic molecules from the cells, formation of protective layer such as biofilm and many others (Munita and Arias 2016). Biofilm formation is one of the most effective and wide spread mechanisms used by several Gram-positive and Gram-negative bacteria to show resistance against common antibiotics. Investigations of new antimicrobials that can work for the inhibition of biofilm formations is becoming a needful strategy to combat antibiotic resistance in clinical cases

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