Abstract
BackgroundGenetic alterations play an important role in the progression of colorectal cancer (CRC). Identifying new biomarkers to assess the prognosis of patients with CRC is critical. Cartilage intermediate layer protein 2 (CILP2) gene, screened from TCGA database by bioinformatics, may be closely related to the progression of CRC. CILP2 was barely reported with clinical features of tumors.Materials and methodsClinical information and RNA-seq data were derived from TCGA colorectal carcinoma cohort. CILP2 expression at mRNA level was estimated by bioinformatical analysis of TCGA cases. Tissue microarray (TMA) was constructed containing paraffin-embedded 64 pairs of CRC and matched adjacent normal tissues. The expression at the protein level was detected in 64 pairs of CRC and matched adjacent normal tissues by immunohistochemical analysis. CILP2 expression level and its clinical value were estimated by bioinformatical analysis with linear and logistic regression. Survival analysis was performed between high and low groups of CILP2 expression by Cox regression analysis, and the P value was calculated by the log-rank test. The Kaplan-Meier curves were tested by the log-rank test.ResultsCILP2 was statistically significantly higher expressed in the CRC tissues when compared with paired adjacent normal tissues in TCGA cohort (P < 0.001) and in the TMA cohort (P = 0.001). Also, CILP2 high expression was strongly correlated with T3/4 stage (P = 0.001), N1/2/3 stage (P = 0.005), M1 stage (P = 0.048), and higher clinical stage (UICC 2010 stage) (P < 0.001) in TCGA cohort, and also positively associated with T3/4 stage (P = 0.022) and higher clinical stage (UICC 2010 stage) (P = 0.03) in TMA cohort. Furthermore, CILP2 overexpression predicted poor prognosis and could be an independent prognostic factor (P = 0.003).ConclusionWe revealed that CILP2 is associated with advanced stages and could play a role as an independent predictor of poor survival in CRC.
Highlights
Colorectal cancer (CRC) is one of the most common cancers that ranks second in cancer-associated mortality in the world, with increasing morbidity in recent years
We evaluated Cartilage intermediate layer protein 2 (CILP2) expression and its correlations with clinicopathological characteristics, such as tumor stages, and overall survival of colorectal cancer (CRC) patients in The Cancer Genome Atlas (TCGA), and furtherly verified using immunohistochemistry assay within human CRC tissues, which may provide a new potential molecular marker for prognostic use of the patients
CILP2 was overexpressed in CRC Aiming at searching for potential novel prognostic markers of CRC, we firstly analyze expression data of TCGA CRC cohort from Illumina HiSeq 2000 platform, which contains 621 samples and correlating clinical and demographic information
Summary
Colorectal cancer (CRC) is one of the most common cancers that ranks second in cancer-associated mortality in the world, with increasing morbidity in recent years. Surgical resection is the mainstay for the treatment of CRC, but tumor recurrence is common. Numerous works have been done to reveal the underlying mechanisms of CRC, and encouraging progress has been made [4,5,6,7]. Further investigating works are still needed to deeply understand the molecular mechanisms, and molecular biomarkers for both early detection and prognosis are to be developed for better therapeutic uses in the patients. Genetic alterations play an important role in the progression of colorectal cancer (CRC). Identifying new biomarkers to assess the prognosis of patients with CRC is critical. Cartilage intermediate layer protein 2 (CILP2) gene, screened from TCGA database by bioinformatics, may be closely related to the progression of CRC.
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