Abstract

The scanning electron microscope (SEM) provides an ideal system for studying the three-dimensional surface structure of biological materials . Our previous work on the protozoan Opalina showed that instantaneous fixation, combined with critical point drying, faithfully preserved the pattern of ciliary coordination and the form of ciliary beat for scanning electron microscopy (1, 2) . I report here new results using these techniques to study ciliary motion in Paramecium multimicronucleatum . In addition, I have compared the suitability of freeze-drying vs . critical point drying as a method for preparing Paramecium for the SEM.

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