Abstract
Results GFP-tagged a-tubulin entered Chlamydomonas cilia as a cargo of IFT and by diffusion. IFT-based transport of GFP-tubulin occurred at a low frequency in full-length steady-state cilia and was strongly increased during ciliary growth when IFT trains carried more tubulin. Cells possessing both non-growing and growing cilia selectively targeted GFP-tubulin into the latter indicating that cells regulate tubulin influx individually for each cilium. The preferential delivery of tubulin boosted the concentration of soluble tubulin in the matrix of growing cilia. Cilia length mutants showed abnormal kinetics of tubulin transport, suggesting that ciliary length control involves a regulation of the occupancy of IFT trains by tubulin cargoes.
Highlights
IFT-based transport of GFP-tubulin occurred at a low frequency in full-length steady-state cilia and was strongly increased during ciliary growth when IFT trains carried more tubulin
The preferential delivery of tubulin boosted the concentration of soluble tubulin in the matrix of growing cilia
Cilia length mutants showed abnormal kinetics of tubulin transport, suggesting that ciliary length control involves a regulation of the occupancy of IFT trains by tubulin cargoes
Summary
Cilium-autonomous regulation of tubulin transport by IFT From Cilia 2014 - Second International Conference Paris, France. Objective The assembly of the axoneme, the structural scaffold of cilia and flagella, requires translocation of a vast quantity of tubulin into the growing cilium, but the mechanisms that regulate the targeting, quantity, and timing of tubulin transport are largely unknown.
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