Abstract
Porcine precision-cut lung slices (PCLS) were used to analyze the effect of the ciliary activity on infection of airway epithelial cells by influenza viruses. Treatment of slices with 2% NaCl for 30 min resulted in reversible ciliostasis. When PCLS were infected by a swine influenza virus of the H3N2 subtype under ciliostatic conditions, the viral yield was about twofold or threefold higher at 24 or 48 h post-infection, respectively, as compared to slices with ciliary activity. Therefore, the cilia beating not only transports the mucus out of the airways, it also impedes virus infection.
Highlights
Porcine precision-cut lung slices (PCLS) were used to analyze the effect of the ciliary activity on infection of airway epithelial cells by influenza viruses
Reversible ciliostasis in differentiated airway epithelial cells To analyze the effect of ciliostasis on virus infection, we chose precision-cut lung slices as a culture system for differentiated airway epithelial cells
The role of the ciliary activity in transporting mucus with entrapped foreign substances out of the airways is a wellrecognized feature of the mucociliary clearance system [2, 3]
Summary
Porcine precision-cut lung slices (PCLS) were used to analyze the effect of the ciliary activity on infection of airway epithelial cells by influenza viruses. We used swine influenza virus of the H3N2 subtype because of its superior growth properties in PCLS [4] to analyze the infection of differentiated respiratory epithelial cells by influenza virus in the absence and presence of ciliostatic conditions. Treatment of PCLS with any of the different hypertonic salt concentrations for 5 min resulted in complete ciliostasis.
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