Abstract
The influence of different hydroxylated additives (methanol, ethyleneglycol, glycerol, erythritol, xylitol, and sorbitol) on the plastein reaction catalyzed by α-chymotrypsin has been studied at different substrate concentrations. The results obtained showed that the increase in the polyol concentration and the number of hydroxylic groups per molecule enhanced the plastein yield. However, when the substrate concentration was also increased, a decrease in this activation effect was observed. In the case of a 3M xylitol solution, the plastein reaction was studied by both quantification of free amino groups and gel-permeation chromatography at different substrate concentrations. The overall analysis of the results allowed us to postulate a schematic integration of all chymotryptic reactions as a general mechanism of the plastein synthesis, as well as to conclude that the presence of additives in the reaction media, as water-activity reducing agents, increase the condensation pathway of the α-chymotrypsin action on the plastein synthesis.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.