Abstract
The influence of different hydroxylated additives (methanol, ethyleneglycol, glycerol, erythritol, xylitol, and sorbitol) on the plastein reaction catalyzed by α-chymotrypsin has been studied at different substrate concentrations. The results obtained showed that the increase in the polyol concentration and the number of hydroxylic groups per molecule enhanced the plastein yield. However, when the substrate concentration was also increased, a decrease in this activation effect was observed. In the case of a 3M xylitol solution, the plastein reaction was studied by both quantification of free amino groups and gel-permeation chromatography at different substrate concentrations. The overall analysis of the results allowed us to postulate a schematic integration of all chymotryptic reactions as a general mechanism of the plastein synthesis, as well as to conclude that the presence of additives in the reaction media, as water-activity reducing agents, increase the condensation pathway of the α-chymotrypsin action on the plastein synthesis.
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