Chrysin in Combination with Insulin Promotes Glucose Uptake in Skeletal Muscle Cell: Impact of Combination Therapy in Diabetes Myopathy (P01-031-19)

Abstract

ObjectivesThe objective of the current study was examining the beneficial effects of DPP4 inhibitor chrysin alone and in combination with insulin benefits diabetes myopathy. MethodsSulforhodamine B (SRB) protein-dye was used to determine the acute toxicity, and 1H NMR spectroscopy was used to identify and quantify glucose levels to assess glucose uptake on treatments chrysin and combination of chrysin with Insulin on differentiated skeletal muscle cells. Pathway analysis was carried out using omics net web server. All experiments were conducted in triplicates, and all data in the graph represent Mean ± S.D. Graph pad software was used to calculate One-way analysis of variance (ANOVA), computed p-value among different groups and values with P < 0.05 is significant. ResultsResults showed that 250 μM chrysin and combination (10 nM Insulin with 250 μM chrysin) treatments are not acutely toxic to skeletal muscle cells and proliferates the cells significant to insulin-treated skeletal muscle cells (Figure 1A). Glucose metabolite levels are studied as skeletal muscle cells adopted cell proliferative and to demonstrate our hypothesis on glucose metabolism. Significant differences were observed in skeletal muscle cells treated with insulin, chrysin, and combination (Figure 1B). It is observed that chrysin alone, combination increases glucose uptake significantly in comparison to control cells. Pathway analysis revealed that GPCR signaling and immune-related signaling plays a role in proliferating skeletal muscle cells to regulate glucose metabolism. ConclusionsThe results of this study propose the use of natural compound chrysin in combination with insulin to promote skeletal muscle health in diabetes mellitus. The combination identified herein must be considered for future therapies to control diabetic myopathy in preclinical and clinical studies. Chrysin can also be applied as a supplement in the diet as well, to control diabetic myopathy. Funding SourcesAuthors wish to express their gratitude to Escuela Nacional de Ciencias Biológicas-Instituto Politécnico Nacional (ENCB-IPN) for providing financial support for the present study. Supporting Tables, Images and/or Graphs▪