Chrysin exerts anti-oxidant properties and restores motor function in MPTP induced mouse model of Parkinson disease

Abstract

Parkinson's disease (PD) is a neurodegenerative disorder. It is characterized by a loss in substantianigra and striatum of dopaminergic neurons. The present study is to measure the antioxidant activity of chrysin and to assess behaviour in MPTP mice model of Parkinson's disease. Methods: Male C57BL/6J mice divided into six groups (n=9). Different concentration (50, 100, 200 mg/kg) of chrysin was administered for five consecutive days, MPTP administered 80 mg/kg (2 X 40 mg/kg 16 h interval) via intraperitoneal. After 48 hours of MPTP injection, a behavioural assessment was performed. After behavioural analysis, animals were sacrificed, and brains were collected. Collected brains were subjected for the analysis of Molecular studies, Biochemistry and Histopathology. Chrysin increased Glutathione peroxidase and Nitric oxide activity compared to MPTP induced mice. Similarly, when compared with the MPTP treated group, TRX 1 also increased in chrysin treated (100 and 200mg/kg) groups. It shows chrysin's antioxidant scavenging activity, and these modifications lead to major behavioural changes in mice treated with chrysin, these results being comparable with standard drug L-Dopa. All the groups were compared with the control groups. The results evidenced that chrysin protects neurons by modulating the antioxidant mechanism against MPTP induced Parkinsonism.