Chryseomicrobium deserti sp. nov., isolated from desert soil in South Korea.

Abstract

A Gram-stain positive, aerobic, non-motile, rod-shaped bacterium (THG-T1.18T) was isolated from desert soil. Growth occurred at 20-35 °C (optimum 28-30 °C), at pH 5-7 (optimum 7) and at 0-4 % NaCl (optimum 0-1 %). Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T1.18T were identified as Chryseomicrobium amylolyticum DSM 23442T (96.6 %), Chryseomicrobium imtechense JCM 16573T (96.3 %) and Chryseomicrobium aureum KACC 17219T (96.1 %). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids and one unidentified glycolipid. The quinone system was composed of MK-7, MK-8 and MK-6. The major fatty acids were iso C15 : 0 and anteiso C15 : 0. The type of peptidoglycan was A4β, containing of l-Orn-D-Glu. The DNA G+C content of strain THG-T1.18T was 50.4 mol%. DNA-DNA hybridization values between strain THG-T1.18T and C. amylolyticum DSM 23442T, C. imtechense JCM 16573T, C. aureum KACC 17219T were 24.7 % (20.1 % reciprocal analysis), 19.5 % (16.1 %) and 10.4 % (6.7 %) respectively. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-T1.18T represents a novel species of the genus Chryseomicrobium, for which the name Chryseomicrobium deserti sp. nov. is proposed. The type strain is THG-T1.18T (=KACC 18929T=CCTCC AB 2016179T).