Chronopharmacological study of furosemide; (VI) influence of prolonged exposure to continuous light

Abstract

We have previously reported that a time-dependent variability is observed in the diuretic effects of furosemide in rats. The present study was undertaken to examine the influence of prolonged exposure to continuous light on chronopharmacological profiles of furosemide in Wistar rats. In study I, rats were maintained for more than 2 weeks under conditions of light (0700–1900 hrs) and dark (1900–0700 hrs) (LD). Furosemide (30 mg/kg) was orally given at 1200 hrs or at 2400 hrs. Urine was collected for 8 hours after the drug and urinary excretion of sodium and furosemide were determined respectively. Thereafter, these rats were exposed to continuous light (LL) for the next 4 weeks, and were again maintained under the LD cycle. The identical trial of study I was repeated at the end of the LL (study II) and the second LD (study III) conditions. Urine volume and urinary excretion of sodium and furosemide following the drug were significantly greater at 1200 hrs than at 2400 hrs under conditions of LD (study I and III). However these administration time-dependent changes in the effects of furosemide and its urinary amount disappeared with LL condition (study II). These findings indicate that the mode of the time-dependent changes in the effects of furosemide is altered by prolonged exposure to continuous light.