Chronomics of Circulating Plasma and Urinary Lipid Peroxides, Antioxidant Enzymes and Other Related Molecules in Cervical Cancer

Abstract

Background: The chronome ( from chronos, time and nomos, rule; time structure ) of lipid peroxidation and antioxidant defense mechanisms may relate to the efficacy and management of preventive and curative chronotherapy. Patients and methods: Newly diagnosed women with cervical carcinoma (N=40), 30 – 60 years of age and age-matched clinically healthy women ( N=30), synchronized for one week with diurnal activity from about 06:00 to about 22:00 and nocturnal rest, provided blood and urine samples every 6 hours for 24-hours under standardized conditions. Breakfast was served around 08:30, lunch around 13:30 and dinner around 20:30. Drugs known to affect the free radical system were not taken. Determinations included plasma lipid peroxides, in the form of malondialdehyde (MDA), blood superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR) activities, plasma ascorbic acid, uric acid, melatonin concentrations and urinary MDA and melatonin levels. Results: A marked circadian variation was demonstrated for each variable in each group by population – mean cosinor (p < 0.01). In addition to anticipated differences in overall mean value (MESOR), patients differed from healthy women also in terms of their circadian pattern. Conclusion: Chronomes of putative antiand pro-oxidants should be mapped to explore their putative chemotherapeutic role as markers in cancer chronoprevention and management of established disease. Chronomics of Circulating Plasma and Urinary Lipid Peroxides, Antioxidant Enzymes and Other Related Molecules in Cervical Cancer R K Singh, Sushma Singh, Ranjana Singh, Seema Mehrotra, Urmila Singh, O. Schwartzkopff, G Cornelissen and Franz Halberg Departments of Biochemistry and Obstetrics & Gynecology, Chhatrapati Shahuji Maharaj Medical University (Formerly King George’s Medical University), India Department of Biochemistry, SGRRIM&HS, India Halberg Chronobiology Center, University of Minnesota, USA doi:10.4172/2155-9929.1000039