Chronic Treatment with NaF Promotes Dyslipidemia in Rats

Abstract

The present study aimed to evaluate the chronic effect of NaF on: 1) inhibitory pathway of insulin signal transduction in liver (L) and muscle (M); 2) plasma concentration (PC) of triglycerides (TGL); 3) total cholesterol (TC); 3) high‐density lipoprotein cholesterol (HDL‐C); 4) low‐density lipoprotein cholesterol (LDL‐C); 5) very low‐density lipoprotein cholesterol (VLDL‐C). Seven‐week‐old castrated male Wistar rats were randomly divided into two groups: 1) control group (CN); 2) fluoride group (FN), which was treated with NaF in drinking water and F in food pellets (4.0 mgF/kg bw/day). After 42 days, PC of TGL, TC, HDL‐C, LDL‐C and VLDL‐C quantification analysis, and insulin receptor substrate‐1 (IRS‐1) serine phosphorylation status tests in L and M were performed by Western Blotting method. The FN group showed a significant increase (p<0.05) in PC of TGL (CN: 30.7 + 3.6 vs FN: 46.9 + 2.7), TC (CN: 76.1 + 2.1 vs FN: 87.0 + 4.2), and VLDL‐C (CN: 6.1 + 0.7 vs FN: 9.4 + 0.5), in comparison with the CN group. However, the treatment with NaF didn't promote alteration in PC of HDL‐C or LDL‐C, or in IRS‐1 serine phosphorylation status in L or in M. The chronic treatment with F promoted: 1) increase in PC of TGL, TC, and VLDL‐C; 2) no alteration in PC of HDL‐C or LDL‐C; 3) no alteration in inhibitory pathway of insulin signal in L or in M. Financial support: FAPESP (2010/10664–5).