Chronic Social Defeat During Adolescence Induces Short- and Long-Term Behavioral and Neuroendocrine Effects in Male Swiss-Webster Mice.

Héctor Miguel Mancha-Gutiérrez,Lilian Mayagoitia-Novales,Carolina López-Rubalcava,Erika Estrada-Camarena,Elena López-Pacheco

doi:10.3389/fnbeh.2021.734054

Abstract

Chronic stress exposure during adolescence is a significant risk factor for the development of depression. Chronic social defeat (CSD) in rodents is an animal model of depression with excellent ethological, predictive, discriminative, and face validity. Because the CSD model has not been thoroughly examined as a model of stress-induced depression within the adolescence stage, the present study analyzed the short- and long-term behavioral and neuroendocrine effects of CSD during early adolescence. Therefore, adolescent male Swiss-Webster (SW) mice were exposed to the CSD model from postnatal day (PND) 28 to PND37. Twenty-four hours (mid-adolescence) or 4 weeks (early adulthood) later, mice were tested in two models of depression; the social interaction test (SIT) and forced swimming test (FST); cognitive deficits were evaluated in the Barnes maze (BM). Finally, corticosterone and testosterone content was measured before, during, and after CSD exposure, and serotonin transporter (SERT) autoradiography was studied after CSD in adolescent and adult mice. CSD during early adolescence induced enduring depression-like behaviors as inferred from increased social avoidance and immobility behavior in the SIT and FST, respectively, which correlated in an age-dependent manner with SERT binding in the hippocampus; CSD during early adolescence also induced long-lasting learning and memory impairments in the Barnes maze (BM). Finally, CSD during early adolescence increased serum corticosterone levels in mid-adolescence and early adulthood and delayed the expected increase in serum testosterone levels observed at this age. In conclusion: (1) CSD during early adolescence induced long-lasting depression-like behaviors, (2) sensitivity of SERT density during normal brain development was revealed, (3) CSD during early adolescence induced enduring cognitive deficits, and (4) results highlight the vulnerability of the adolescent brain to social stressors on the adrenal and gonadal axes, which emphasizes the importance of an adequate interaction between both axes during adolescence for normal development of brain and behavior.

Highlights

During adolescence, chronic stress exposure can increase the susceptibility to develop various psychiatric disorders, such as depression (Lupien et al, 2009)
The Chronic Social Defeat Model During Early Adolescence Induced Enduring Depression-Like Behaviors Figure 1A shows the effect of Chronic social defeat (CSD) during early adolescence on the social interaction ratio in the SIT in both adolescent and adult mice
Our results show that exposure to CSD during early adolescence induces depression-like behaviors in mid-adolescence and early adulthood

Summary

Introduction

Chronic stress exposure can increase the susceptibility to develop various psychiatric disorders, such as depression (Lupien et al, 2009). Adolescence is the transition stage between childhood and adulthood, which is characterized by significant changes at hormonal, somatic, and behavioral levels, as well as a continuous neurodevelopmental process, which collectively lead to reproductive efficiency and social and cognitive maturation of the individual (Lupien et al, 2009; Holder and Blaustein, 2014). Bullying during adolescence has become a significant risk factor for several psychiatric disorders, such as depression (Iñiguez et al, 2016; Resende et al, 2016)

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