Chronic nitrogen addition induces a cascade of plant community responses with both seasonal and progressive dynamics

Abstract

Short-lived herbaceous plants provide a useful model to rapidly reveal how multiple generations of plants in natural plant communities of sensitive desert ecosystems will be affected by N deposition. We monitored dynamic responses of community structure, richness, evenness, density and biomass of herbaceous plants to experimental N addition (2:1 NH4+:NO3− added at 0, 0.5, 1, 3, 6 and 24gNm−2a−1) in three seasons in each of three years in the Gurbantunggut desert, a typical temperate desert of central Asia. We found clear rate-dependent and season-dependent effects of N deposition on each of these variables, in most cases becoming more obvious through time. N addition reduced plant richness, leading to a loss of about half of the species after three generations in the highest N application level. Evenness and density were relatively insensitive to all but the greatest levels of N addition for two generations, but negative effects emerged in the third generation. Biomass, both above and below ground, was non-linearly affected by N deposition. Low and intermediate levels of N deposition often increased biomass, whereas the highest level suppressed biomass. Stimulatory effects of intermediate N addition disappeared in the third generation. All of these responses are strongly interrelated in a cascade of changes. Notably, changes in biomass due to N deposition were mediated by declines in richness and evenness, and other changes in community structure, rather than solely being the direct outcome of release from limitation. The interrelationships between N deposition and the different plant community attributes change not only seasonally, but also progressively change through time. These temporal changes appear to be largely independent of interannual or seasonal climatic conditions.