Chronic exposure to dietary antibiotics affects intestinal health and antibiotic resistance gene abundance in oriental river prawn (Macrobrachium nipponense), and provokes human health risk

Abstract

Antibiotics are employed worldwide to treat diseases of humans and other animals, but most antibiotics and their secondary metabolites are discharged into the aquatic environment, and these pollutants are emerging as a severe threat to human health. However, the systematic effects of dietary antibiotics on intestinal health in crustaceans and the associated risk to human health have not been thoroughly investigated, especially the effects on growth, immune responses, intestinal health, and the abundance of antibiotic residues and antibiotic resistance genes (ARGs). In the present work, two typical antibiotics (sulfamethoxazole and oxytetracycline) were administered orally to juvenile oriental river prawn (Macrobrachium nipponense) for eight weeks to mimic long-term use of antibiotics at legal aquaculture doses. The results indicate that dietary exposure to antibiotics significantly inhibited the growth performance of prawns, suppressed immunological parameters, and caused higher mortality in prawns challenged with Aeromonas hydrophila. Furthermore, prawns fed a diet containing antibiotics displayed a decrease in the number of intestinal goblet cells and lower digestive enzyme activity, as well as impaired intestine antioxidant ability and immune responses. Additionally, redundancy analysis revealed that different dominant bacterial phyla were responsible for increased ARG abundance in the prawn intestinal tract between control and antibiotic diet groups. The health risks posed by antibiotic residues in prawn muscle were also evaluated. Our findings demonstrate the risk posed by long-term use of dietary antibiotics in prawns, and suggest that antibiotics should be administered more carefully during aquaculture.