CHRONIC CONSUMPTION OF NON‐CALORIC SWEETENERS IN CARBONYLATED PROTEIN CONCENTRATIONS FROM BLOOD LYMPHOCYTES OF CD1 MICE

Abstract

The consumption of non‐caloric sweeteners as a strategy to reduce energy intake has recently increased. To our knowledge, there's not studies about protein carbonylation in lymphocytes based in sweeteners consumption. The aim of this study was to compare the effect of chronic consumption of non‐caloric sweeteners in carbonylated protein concentrations from blood lymphocytes of CD1 mice. We used 32 63‐day old CD1 mice, divided into 4 groups: Control (Cl), Sucrose (Suc), Sucralose (SUC), and Stevia (STE). 10g of SAC and 1g of SUC and ST were diluted in 240mL of ultrapure water, mice drank and fed ad libitum. Sweeteners were administrated for 6 weeks, after that, the mice were sacrificed by cervical dislocation and blood obtained by cardiac puncture. Lymphocytes were isolated and 2–4 Dinitrophenylhydrazine assay for quantification of protein carbonylation was performed. There was a significant difference between study groups (F=4.93, p<0.01). Carbonylated protein concentrations in SUC was significantly higher than SAC (Bonferroni test p<0.001) but ST was significantly lower than SUC (Bonferroni test p<0.01) indicating that chronic consumption of SUC may be was associated with increased protein carbonylation in blood lymphocytes.Support or Funding InformationUAEM