Chronic cholestasis is associated with hypogonadism and premature ovarian failure in adult rats (cholestasis causes ovarian hypogonadism)

Abstract

ABSTRACTHypogonadism is a well-known complication in males with chronic liver diseases. However, the consequences of chronic hepatopathies on female reproductive capacities have received relatively little attention. The present study evaluates the effect of chronic obstructive jaundice on the ovary of adult cycling rats. Estrous cyclicity was monitored to check the functional status of the hypothalamic–pituitary–ovarian axis. Ovarian changes were assessed using histomorphometric, immunohistochemical, and ultrastructural techniques. Chronic cholestasis was associated with estrous cycle irregularities, diminished ovarian weight, primordial follicle loss, atretic follicle prevalence, marked stromal fibrosis, and diminished immunoexpression of proliferation marker and estrogen receptors, in addition to many ultrastructural alterations in theca, granulosa cells, and oocytes of antral follicles. The results establish that chronic cholestasis causes hypogonadism and premature ovarian insufficiency in adult cycling female rats.