Chromosomes of Pacific hydrothermal vent invertebrates: towards a greater understanding of the relationship between chromosome and molecular evolution

Abstract

Karyotypes for several East Pacific Rise hydrothermal vent invertebrates are described here for the first time: the vestimentiferansRiftia pachyptilaandOasisia alvinae, the alvinellid polychaetesAlvinella pompejana, A. caudataandParalvinella grasslei, the polynoid polychaetesBranchinotogluma grassleiandBranchipolynoe symmytilida, the serpulidLaminatubus alviniand the mytilid bivalveBathymodiolus thermophilus. For comparative purposes, the karyotype of the Atlantic vent musselBathymodiolus azoricusis also described here for the first time. Each species has its own unique chromosomal characteristics which can be interpreted both in terms of group characteristics and species divergence. From comparisons with published results on other vent species and closely-related coastal species, we identified a positive correlation between chromosome number variation and molecular divergence at two ribosomal ribonucleic acid gene loci (the 18S and 28S rRNA). Whilst the patterns of chromosome divergence we found were generally within the ranges previously reported for these taxonomic groupings, there was an apparent inconsistency in the case ofBranchipolynoe symmytilida(EPR) andBranchipolynoe seepensis(MAR), which show a greater degree of divergence at the chromosome level compared with other members of the same genus. Moreover, polychaetes as a whole showed greater variation in the number and structural divergence of chromosomes compared to Mytilids (structural information only). Our findings highlight the great potential for chromosome analysis in future taxonomic and evolutionary studies of the deep-sea vent fauna.