Abstract

Meiotic prophase is classically subdivided into five stages: leptotene, zygotene, pachytene, diplotene, and diakinesis. The objective of this paper is to evaluate the behaviours of Chromosomes in Chlorophytum stenopetalum Bak.at pachytene /diakinesis and metaphase. The flower buds at right age were harvested, fixed in Cornoy’s solution (3 part of absolute alcohol and 1 part of acetic alcohol) and preserved in a refrigerator at -4°𝐶 for at least thirty minutes. The flower buds were then hydrolyzed in 10% HCl for 3-5 minutes. Prepared slides were viewed using an Armscope microscope equipped with digital automatic camera. At diakinesis seven bivalents (7 II) were predominantly observed (74.2%). Chromosomal stickiness is quite evidence. In addition, cross configuration and its resultant, ring formation at metaphase indicate the presence of translocation heterozgosity in the chromosomes of the species investigated. These abnormalities are likely to affect microsporogenesis and pollen viability.

Highlights

  • Meiotic prophase is classically subdivided into five stages: leptotene, zygotene, pachytene, diplotene, and diakinesis

  • Chlorophytum Ker-Gawl is a genus in the family Asperagaceace, sub-family Agavoideae of monocotyledonous perennial flowering plant, order Asparagales (Angiosperm Phylogeny Group IV, 2016)

  • Chlorophytum stenopetalum is found in the guinea savanna region in Nigeria

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Summary

Introduction

Meiotic prophase is classically subdivided into five stages: leptotene, zygotene, pachytene, diplotene, and diakinesis. The objective of this paper is to evaluate the behaviours of Chromosomes in Chlorophytum stenopetalum Bak.at pachytene /diakinesis and metaphase. Cross configuration and its resultant, ring formation at metaphase indicate the presence of translocation heterozgosity in the chromosomes of the species investigated. These abnormalities are likely to affect microsporogenesis and pollen viability. Chlorophytum Ker-Gawl is a genus in the family Asperagaceace, sub-family Agavoideae of monocotyledonous perennial flowering plant, order Asparagales (Angiosperm Phylogeny Group IV, 2016). This position stated in Angiosperm Phylogenic Group III, 2009 is maintained the new classification (Steven, 2007). Chlorophytum stenopetalum is sometimes confused with Chlorophytum macrophyllum, Omokanye et al (2020), separated the two based on their leaf

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