Abstract
The transplantability to adult hamsters, the CF titers of tumour antigen and the chromosome content was determined at a single passage level for two established adenovirus 12-induced hamster tumour cell lines and an SV40-virus tumour cell line. The cell lines had been passaged more than 200 timesin vitro, and immunofluorescent tests showed that all the cells contained specific virusinduced tumour antigen. The SV40 cell line and one of the adenovirus 12 tumour cell lines (TAd III cells) were transplantable to adult hamsters. The second adenovirus 12 tumour cell line (H212 cells) did not produce tumours in adult hamsters, but produced tumours in new-born hamsters. TAd III cells contain relatively a low CF titre of tumour antigen while H212 cells contain high CF titres of antigen. Cytogenetic analysis showed that all tumour cell lines were markedly different from normal hamster cells. The incidence of marker chromosomes was greatest for SV40 tumour cells, and smallest for H212 cells. The results of cytogenetic and antigen studies are discussed in relation to the transplantability of the tumour cell lines.
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