Abstract
Intergeneric hybrids between Saccharum spp. and Erianthus arundinaceus and clones derived from these hybrids and backcrosses to Saccharum spp. were used to study the transmission of E. arundinaceus chromosomes by genomic in situ hybridization (GISH). True hybrid progenies were precisely identified using PCR with a primer pair, AGRP52/53. The results showed that AGRP52/53 was an E. arundinaceus-specific primer pair and could be used as molecular marker to assist breeding. EaHN92, a 364 bp E. arundinaceus-specific tandem repeat satellite DNA sequence, was cloned from the E. arundinaceus clone HN92–105 with AGRP52/53, and was localized on sub-telomeric regions of all E. arundinaceus chromosomes. YCE06–61, a BC3 progeny, had 7 E. arundinaceus chromosomes and its progenies had approximately 1–6 E. arundinaceus chromosomes. The number of E. arundinaceus chromosomes in true hybrids appeared as Gaussian distribution in 3 cross combinations. In addition, GISH detected intergeneric chromosome translocation in a few progenies. Hence, screening clones containing approximately 1–2 E. arundinaceus chromosomes without translocation could be used for sorting and sequencing E. arundinaceus chromosomes. This study provides a method for breeders to select true hybrid progenies between Saccharum spp. and E. arundinaceus, which will accelerate this intergeneric hybridization breeding.
Highlights
Intergeneric hybrids between Saccharum spp. and Erianthus arundinaceus and clones derived from these hybrids and backcrosses to Saccharum spp. were used to study the transmission of E. arundinaceus chromosomes by genomic in situ hybridization (GISH)
In 1996, D’Hont et al.[3] reported that modern sugarcane cultivars possess approximately 120 chromosomes, with 70–80% derived from S. officinarum, 10–20% derived from S. spontaneum, and a few chromosomes derived from interspecific recombination
The repeat units of EaHN92 tandem repeat sequences was 364 bp satellite DNA sequence, which was cloned from E. arundinaceus HN92–105
Summary
Intergeneric hybrids between Saccharum spp. and Erianthus arundinaceus and clones derived from these hybrids and backcrosses to Saccharum spp. were used to study the transmission of E. arundinaceus chromosomes by genomic in situ hybridization (GISH). Repetitive sequences are sources of molecular markers that are useful in plant genetic studies, and have been cloned from many higher plant genera for use in phylogenetic studies or introgression breeding programs[10,11,12,13] They diverged rapidly during evolution and are constantly homogenized, giving rise to sequences that are species-specific, genus-specific, and even chromosome-specific[14,15]. Their localization by situ hybridization provide important information on chromosome structure[16] These repetitive DNA sequences have provided new molecular tools to investigating genetic diversity and phylogenetic relationships in the Saccharum complex and improving the efficiency of modern molecular breeding of sugarcane
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