Abstract
We provide the raw and processed data produced during the genome sequencing of isolates from six species of parasites from the sub-family Leishmaniinae: Leishmania martiniquensis (Thailand), Leishmania orientalis (Thailand), Leishmania enriettii (Brazil), Leishmania sp. Ghana, Leishmania sp. Namibia and Porcisia hertigi (Panama). De novo assembly was performed using Nanopore long reads to construct chromosome backbone scaffolds. We then corrected erroneous base calling by mapping short Illumina paired-end reads onto the initial assembly. Data has been deposited at NCBI as follows: raw sequencing output in the Sequence Read Archive, finished genomes in GenBank, and ancillary data in BioSample and BioProject. Derived data such as quality scoring, SAM files, genome annotations and repeat sequence lists have been deposited in Lancaster University’s electronic data archive with DOIs provided for each item. Our coding workflow has been deposited in GitHub and Zenodo repositories. This data constitutes a resource for the comparative genomics of parasites and for further applications in general and clinical parasitology.
Highlights
Background & SummaryLeishmaniasis is a neglected tropical disease
It is considered to be a disease of poverty, primarily affecting low and middle-income countries (LMICs)
Leishmaniasis is caused by parasites of the genus Leishmania and 18 different species are known to infect humans1. 98 sandfly species are suspected or confirmed vectors of Leishmania[2]
Summary
Leishmaniasis is caused by parasites of the genus Leishmania and 18 different species are known to infect humans. Mundinia, the latter accommodating several species from the L. enriettii complex and others, from five continents[6,7,8,9,10,11,12]. Mundinia subgenus genomes have been sequenced and assembled: Leishmania enriettii, strain LEM3045 (GCA_000410755) and Leishmania sp. Prior to the release of our genome, there were no genome sequences for genus Porcisia. Namibia - and one genome in the genus Porcisia – that of P. hertigi, formerly known as L. The two isolates from Ghana and Namibia are from new species that have not yet been formally named. This workflow generated four main outputs for each assembly: genome, proteome, and transcriptome files in FASTA format, and a General Feature Format file (GFF) that contains the coordinates for all proteins and transcripts in the assembly
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