Abstract
ABSTRACTLeishmania (Mundinia) orientalis is a kinetoplastid parasite first isolated in 2014 in Thailand. We report the complete genome sequence of L. (M.) orientalis, sequenced using combined short-read and long-read technologies. This will facilitate greater understanding of this novel pathogen and its relationship to other members of the subgenus Mundinia.
Highlights
Leishmaniasis is spread through sand fly bites and caused by kinetoplastid parasites of the genus Leishmania [1]
Using Minimap2 [14] and SAMtools [15], we mapped the short reads onto the assembled scaffolds to correct erroneous bases within the long reads and create consensus sequences
We removed the duplicated contigs and sorted the remainder according to length using Funannotate [17, 18]
Summary
Leishmaniasis is spread through sand fly bites and caused by kinetoplastid parasites of the genus Leishmania [1]. Short-read library construction and sequencing were contracted to (i) BGI (Shenzhen, China) for DNBSEQ libraries, producing paired-end reads (170 bp, 270 bp, and 500 bp) using the Illumina HiSeq platform, and (ii) Aberystwyth University (Aberystwyth, UK) for TruSeq Nano DNA libraries, producing paired-end reads (300 bp) using the Illumina MiSeq platform. We performed long-read library preparation and sequencing according to the Nanopore protocol (SQK-LSK109) on R9 flow cells (FLO-MIN106).
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