Abstract
In this study, the intergeneric hybrids F1, F2, BC1F1, BC1F2, and BC2F1 from Elytrigia elongata and Triticum aestivum crosses were produced to study their chromosome pairing behavior. The average E. elongata chromosome configuration of the two F1 hybrids agreed with the theoretical chromosome configuration of 21I+7II, indicating that the genomic constitution of this F1 hybrid was ABDStStEeEbEx. Compared with the BC1F1 generation, the BC2F1 generation showed a rapid decrease in the number of E. elongata chromosomes and the BC1F2 generation showed a more extensive distribution of E. elongata chromosomes. In addition, pairing between wheat and E. elongata chromosomes was detected in each of the wheat-E. elongata hybrid progenies, albeit rarely. Our results demonstrated that genomic in situ hybridization (GISH) using an E. elongata genomic DNA probe offers a reliable approach for characterizing chromosome pairing in wheat and E. elongata hybrid progenies.
Highlights
Modern cultivation strategies have diminished the genetic base of common wheat (Triticum aestivum)
The F1 hybrids from the E. elongata × T. aestivum cross exhibited a low setting percentage and were morphologically different from the 2 parents, except for a similar perennial of E. elongata
Meiotic association was determined in 29 pollen mother cells (PMCs) at the Metaphase I (MI) stage from E. elongata × T. aestivum cv
Summary
Modern cultivation strategies have diminished the genetic base of common wheat (Triticum aestivum). Thinopyrum ponticum (Podp.) Barkworth] (2n = 10x = 70) was initially hybridized with wheat approximately 70 years ago because of its resistance to several wheat diseases, as well as its stress tolerance and high crossing ability with various Triticum species (Sepsi, 2010; Hu et al, 2011; Fu et al, 2012; Ayala-Navarrete et al, 2013; He et al, 2013; Zheng et al, 2014; Li et al, 2016) Many desirable genes, such as Sr25, Sr43, Lr19, Cmc, and Pm51, have been characterized and transferred from this wild grass species into wheat. The genomic composition of E. elongata has not yet been clarified
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