Abstract
Additional evidence has been obtained that demonstrates the low capacity of ascidians to polyploidize. The evolution of ascidians appears to have been accompanied by a decrease of chromosome number within the Phlebobranchiata, and by both a decrease and increase of chromosome number in the other two suborders, Aplousobranchiata and Stolidobranchiata. There are indications that the basic chromosome number, 2n=32, of Stolidobranchiata is of very early origin within the class Ascidiacea.
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