Abstract
Chromosome-sized DNA molecules from Leishmania isolates ( L. mexicana amazonensis, L. mexicana mexicana, L. chagasi, L. major, L. donovani, and L. braziliensis) were separated by orthogonal field alternation gel electrophoresis. The chromosome locations of four genes were mapped. The α-tubulin and rRNA genes each mapped to a single chromosome size class. The β-tubulin and the 5′-spliced-leader-sequence genes were found on more than one chromosome size class and showed variation of hybridization profiles across species.
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