Abstract
The humpback puffer, Tetraodon palembangensis, is a poisonous freshwater pufferfish species mainly distributed in Southeast Asia (Thailand, Laos, Malaysia and Indonesia). The humpback puffer has many interesting biological features, such as inactivity, tetrodotoxin production and body expansion. Here, we report the first chromosome-level genome assembly of the humpback puffer. The genome size is 362Mb, with a contig N50 value of ∼1.78Mb and a scaffold N50 value of ∼15.8Mb. Based on this genome assembly, ∼61.5Mb (18.11%) repeat sequences were identified, 19,925 genes were annotated, and the function of 90.01% of these genes could be predicted. Finally, a phylogenetic tree of ten teleost fish species was constructed. This analysis suggests that the humpback puffer and T. nigroviridis share a common ancestor 18.1 million years ago (MYA), and diverged from T. rubripes 45.8 MYA. The humpback puffer genome will be a valuable genomic resource to illustrate possible mechanisms of tetrodotoxin synthesis and tolerance.
Highlights
The humpback puffer, Tetraodon palembangensis (NCBI Taxonomy ID: 1820603, Fishbase ID: 25179), known as Pao palembangensis, is widely distributed in Southeast Asia and prefers to live in alkalescent, warm (24–28°), and slow-flowing rivers (Figure 1a) [1]
The wild population of humpback puffer has declined in recent years owing to the destruction of suitable habitat caused by climate change and overfishing [4]
A paired-end single tube long fragment reads library and an RNA library were constructed according to the protocol published by Wang et al [10]
Summary
The humpback puffer, Tetraodon palembangensis (NCBI Taxonomy ID: 1820603, Fishbase ID: 25179), known as Pao palembangensis, is widely distributed in Southeast Asia and prefers to live in alkalescent, warm (24–28°), and slow-flowing rivers (Figure 1a) [1]. The wild population of humpback puffer has declined in recent years owing to the destruction of suitable habitat caused by climate change and overfishing [4]. With these biological characteristics and a small genome size, the humpback puffer is an ideal species for genetic study [5]. It is a species in the Fish10K Genome Project, a subproject of the Earth BioGenome Project, which aims to sample, sequence, assemble and analyse the genomes of 10,000 fish species [6].
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