Abstract
The Rock Bream (Oplegnathus fasciatus) is an economically important rocky reef fish of the Northwest Pacific Ocean. In recent years, it has been cultivated as an important edible fish in coastal areas of China. Despite its economic importance, genome-wide adaptions of domesticated O. fasciatus are largely unknown. Here we report a chromosome-level reference genome of female O. fasciatus (from the southern population in the subtropical region) using the PacBio single molecule sequencing technique (SMRT) and High-through chromosome conformation capture (Hi-C) technologies. The genome was assembled into 120 contigs with a total length of 732.95 Mb and a contig N50 length of 27.33 Mb. After chromosome-level scaffolding, 24 chromosomes with a total length of 723.22 Mb were constructed. Moreover, a total of 27,015 protein-coding genes and 5,880 ncRNAs were annotated in the reference genome. This reference genome of O. fasciatus will provide an important resource not only for basic ecological and population genetic studies but also for dissect artificial selection mechanisms in marine aquaculture.
Highlights
The Rock Bream (Oplegnathus fasciatus), inhabiting in coastal rocky reefs and feeds on invertebrates inhabiting the seabed, is an endemic marine fish in East Asia that belongs to Oplegnathidae in Perciformes (Figure 1A) (An et al, 2008)
The genome size is slightly smaller than that of the O. fasciatus derived from the northern population
We obtained a final genome assembly of 732.95 Mb for the O. fasciatus, which is nearly equal to the estimated genome size (Table1 and Supplementary Table S1)
Summary
The Rock Bream (Oplegnathus fasciatus), inhabiting in coastal rocky reefs and feeds on invertebrates inhabiting the seabed, is an endemic marine fish in East Asia that belongs to Oplegnathidae in Perciformes (Figure 1A) (An et al, 2008). O. fasciatus has become one of the most commercially valuable marine fishery species in China aquaculture (Xiao et al, 2019). Rock Bream Chromosome-Level Genome Assembly species, due to habitat restrictions, O. fasciatus is broadly and fragmented distributed in the coastal areas of eastern China (Xiao et al, 2016a). According to the difference of genetic variation, the researchers identified the O. fasciatus population as northern (Jiaonan, Qingdao) and southern regions (Zhoushan, Zhejiang) of Chinese coastal waters (Xiao et al, 2016b). A highly accurate reference genome of subtropical O. fasciatus species is still lacking, which hinders the progress of genome-scale genetic breeding and genetic studies of its temperature plasticity and adaptation at lower latitudes
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