Abstract
Characterization of all chromosomes of the Andean G19833 bean genotype was carried out by fluorescent in situ hybridization. Eleven single-copy genomic sequences, one for each chromosome, two BACs containing subtelomeric and pericentromeric repeats and the 5S and 45S ribosomal DNA (rDNA) were used as probes. Comparison to the Mesoamerican accession BAT93 showed little divergence, except for additional 45S rDNA sites in four chromosome pairs. Altogether, the results indicated a relative karyotypic stability during the evolution of the Andean and Mesoamerican gene pools of P. vulgaris.
Highlights
The common bean (Phaseolus vulgaris L.) is one of the main legume crops worldwide, with special dietary value for Latin America and Africa, where it represents a major source of proteins (Broughton et al, 2003)
Due to its distribution in the wild and two major domestication centers, P. vulgaris germplasm is comprised of two main gene-pools, the Andean and the Mesoamerican (Singh et al, 1991)
A large BAC library exists for this Mesoamerican cultivar (Kami et al, 2006) and clones from this library were used for establishing the cytogenetic map of the common bean integrated to its genetic maps (Pedrosa-Harand et al, 2009; Fonsêca et al, 2010)
Summary
The common bean (Phaseolus vulgaris L.) is one of the main legume crops worldwide, with special dietary value for Latin America and Africa, where it represents a major source of proteins (Broughton et al, 2003). Other repetitive sequences present in BAC inserts and localized in BAT93 (Pedrosa-Harand et al, 2009; Fonsêca et al, 2010) have not been analyzed in Andean accessions. Three out of four BACs defined for karyotyping in common bean contained repetitive sequences that may vary in distribution between gene pools, hampering chromosome identification.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
