Abstract
PAINTER1 and Schrader2 have recently suggested that the long chromosomes of the germ-line cells in Ascaris megalocephala are peculiar in having a large number of spindle attachments instead of only one as hitherto assumed. Some time ago, I carried out an experiment to test this hypothesis. Uteri of adult females (var. bivalens) were irradiated with a Coolidge tube (65 kv., 5 ma., 30 cm. distance, unscreened radiation for 5 minutes). They were then kept for 5½ days at 38° C. and fixed in Carnoy. In several cases the long chromosomes of the first cleavage division were fragmented as a result of the irradiation. Fig. 1 shows a cell in which one of the four chromosomes has been broken in two places, leaving three fragments which are all attached to the spindle.
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