Abstract
Mutagenic studies have been made after single and combined treatments of gamma rays, ethylmethane sulphonate (EMS) and hydroxylamine (HA) in Catharanthus roseus of pink flowered form (PFF). The present observations in meiotic system disclosed various types of chromosomal alterations such as different association of chromosomes at diakinesis, unequal disjunction at anaphase I, unoriented chromosomes at metaphase I and II, fragments, bridges with or without laggards, and micronuclei at telophase II. Besides, tetrad abnormalities like monads, dyads, triads and polyads were observed. Similar types of meiotic anomalies were also recorded in mutants like sterile (in which flower modified into vegetative structures), variegated, non-branching and dissected flower. The abnormal phenotype of the above mutants may be attributed to chromosomal alterations. The combination treatments proved to be much effective than individual treatments.
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