Abstract
Purpose : To determine whether measurement of chromosome aberrations by fluorescence in situ hybridization (FISH) predicts cell survival after irradiation at different dose-rates and after radiosensitization by bromodeoxyurdine (BrdU) in a lung carcinoma cell line. Materials and methods : The human lung carcinoma cell line SW1573 was irradiated at high dose-rate (HDR: 0.8 Gy min -1) or at pulsed low dose-rate (p-LDR: average dose-rate 1 Gy h -1) with or without radiosensitization by bromodeoxyuridine (BrdU). Cell survival was determined by clonogenic assay. Chromosome aberrations (colour junctions) were measured by whole-chromosome FISH of chromosome 2 and 18 and were scored according to the PAINT method. Results : Clear radiosensitization by BrdU was observed both after HDR and p-LDR irradiation. Chromosome 18 was more radiosensitive than chromosome 2. There was a good correlation between induction of colour junctions and cell survival both after HDR and p-LDR irradiation and after radiosensitization by BrdU. Conclusions : Determination of chromosome aberrations by FISH can predict cell survival after different dose-rates and after radiosensitization by BrdU
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