Chromosome Aberrations as a Biomarker for Genomic Instability in Cell Cultures Originated From Bovines, Canines and Equines Infected With Papillomavirus

Ortrud Monika Barth,Rachel Siqueira De Queiroz Simões

doi:10.3126/ijasbt.v4i1.14727

Abstract

Chromosomal abnormalities or aberrations were investigated in ten bovines, three canines and three equines presenting cutaneous papillomatosis. The aim of the present study was to evaluate possible chromosomal abnormalities as a biomarker of genomic instability induced by the interaction of the viral genome with the chromatin of the host cell in peripheral blood lymphocytes and skin warts lesion cultures. Genomic DNA was extracted from blood samples and epithelial biopsies. Amplification products from PCR using degenerated primer pair FAP59/FAP64 were sequenced. Peripheral blood lymphocytes and tumor cell cultures were performed. Structural chromosomal aberrations were verified as associations, chromosomal fragments, centric fusions, open chromatids, gaps, rearrangements and rings. Several levels of premature chromosome condensation were observed in both cultures. Numeric abnormalities as aneuploidy were also detected and hipoployd cells were found in a larger number than the hiperployd cells. This cytogenetic study in detecting premature chromosome condensation in the three species investigated describes by the first time the structural and numerical abnormalities in canines and equines presenting papillomatosis lesions. These abnormalities are similar of the three groups of animals, but virus specific.Int J Appl Sci Biotechnol, Vol 4(1): 104-112

Highlights

The papillomaviruses belong to the Papillomaviridae family and new genera have been described on the basis of the interspecies phylogenetic similarity
The aim of the present study is to evaluate possible chromosomal abnormalities induced by the interaction of the viral genome with the chromatin of the host cell in peripheral blood lymphocytes and skin wart lesion cultures of bovines, canines and equines infected with papillomavirus
Samples sequenced of the Bovine papillomavirus (BPV) and canine oral papillomavirus (COPV) showed similarity with the sequences of the HPV by molecular analysis

Summary

Introduction

The papillomaviruses belong to the Papillomaviridae family and new genera have been described on the basis of the interspecies phylogenetic similarity. Previous studies have reported the occurrence of chromosomal aberrations in peripheral blood lymphocytes cultured from cattle infected by Bovine papillomavirus (BPV) after consumption or not of the carcinogenic substances of the fern Pteridium aquilinum, regardless of the development of bovine enzootic hematuria (Stocco dos Santos et al, 1998). Presence of DNA sequences of the Bovine papillomavirus 2 (BPV-2) was detected in the cattle with bladder cancer. BPV DNA sequences have been detected in peripheral blood of non-clinically affected cattle by papillomatosis, presenting high levels of chromosomal aberrations or abnormalities (Campos et al, 1994; Stocco dos Santos et al, 1998). Chromosomal fragility has been detected in lymphocytes of women with lesions in the cervix after submitted to a treatment against HPV (Paz-y-mino et al, 1992)

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