Chromosomal localization of omega and trophoblast interferon genes in cattle and river buffalo by sequential R-banding and fluorescent in situ hybridization.

Abstract

Fluorescent in situ hybridization (FISH) of the cattle cDNA probe bTP-509 to RBA-banded cattle (Bos taurus L.) chromosomes confirmed the assignment of the omega (IFNW) and trophoblast (IFNT) interferon genes to chromosome 8q15. Using the same probe, these genes were also localized to river buffalo (Bubalus bubalis L.) chromosome 3q15 following sequential RBA-banding and FISH. The extensive G- and R-banding homology observed between cattle chromosome 8 and river buffalo chromosome arm 3q supports the conserved chromosomal location of the IFNW and IFNT genes in these two species.