Abstract
Creation of wheat-alien disomic addition lines and localization of desirable genes on alien chromosomes are important for utilization of these genes in genetic improvement of common wheat. In this study, wheat-Agropyron cristatum derivative line 5113 was characterized by genomic in situ hybridization (GISH) and specific-locus amplified fragment sequencing (SLAF-seq), and was demonstrated to be a novel wheat-A. cristatum disomic 6P addition line. Compared with its parent Fukuhokomugi (Fukuho), 5113 displayed multiple elite agronomic traits, including higher uppermost internode/plant height ratio, larger flag leaf, longer spike length, elevated grain number per spike and spikelet number per spike, more kernel number in the middle spikelet, more fertile tiller number per plant, and enhanced resistance to powdery mildew and leaf rust. Genes conferring these elite traits were localized on the A. cristatum 6P chromosome by using SLAF-seq markers and biparental populations (F1, BC1F1 and BC1F2 populations) produced from the crosses between Fukuho and 5113. Taken together, chromosomal localization of these desirable genes will facilitate transferring of high-yield and high-resistance genes from A. cristatum into common wheat, and serve as the foundation for the utilization of 5113 in wheat breeding.
Highlights
Common wheat (Triticum aestivum, genome AABBDD) has a large number of wild relatives, which are considered as valuable gene resources for wheat genetic improvement [1]
Wheatalien disomic addition lines are usually considered as key materials to transfer alien superior genes into common wheat, which play important roles in numerous applications, such as investigating genetic effects of alien chromosomes under wheat background, determining chromosomal localization of some desirable genes, purifying alien chromosomes or chromosome
Twenty-one wheat bivalents and one A. cristatum bivalent were shown in Fig 1B, showing regular chromosome pairing behaviors. All these results indicated that 5113 was a stable wheat-A. cristatum disomic addition line
Summary
Common wheat (Triticum aestivum, genome AABBDD) has a large number of wild relatives, which are considered as valuable gene resources for wheat genetic improvement [1]. Creation of wheat-alien disomic addition lines and chromosomal localization of their elite genes are important for wheat genetic improvement. Plenty of wheat-alien addition lines derived from multiple species of Triticeae have been obtained. Most wheat-alien disomic addition lines could not be directly used in wheat breeding due to the presence of linkage drag. They are usually induced to produce various translocation or introgression lines, such as wheat-Psathyrostachys huashanica translocation line H9020-17-15 [14], wheat Haynaldia villosa translocation line 6VS/6AL [15], wheat-rye translocation line 1BL/1RS [16], and wheat-Thinopyrum pointicum introgression lines II-1-8 and II-2 [17]. Before addition lines are induced to produce translocation or introgression lines, localization of genes conferring elite agronomic traits were usually considered as a fundamental prerequisite
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