Chromosomal Localization and Contribution of Three Homoeologous Genes to Biosynthesis of Cytosolic Aspartate Aminotransferase in Common Wheat.

Marcin Maciąga,Michał Szkop,Andrzej Paszkowski

doi:10.1007/s40011-015-0536-7

Abstract

Chromosomal localization of the three homoeologous genes encoding cytosolic aspartate aminotransferase in common wheat (Triticum aestivum cv. Chinese Spring, 2n = 6x = 42, AABBDD) was specified to: 3AL (0.42÷0.61), 3BL (0.38÷0.41) and 3DL (0.23÷0.81) by a comparative zymographic analysis of the enzymatic activities in deletion lines. It was also attempted to precisely explain the nature of the relationship between a number of genes encoding α and β subunits and a distribution of staining intensity of cytosolic aspartate aminotransferase allozyme activity bands using aneuploid lines of common wheat with modified third pair of homoeologous chromosomes from genomes A, B and D, on which the genes encoding subunit α (genome A) and β (genome B and D) are localized. The highest consistency between the experimental results and the theoretical distributions was achieved by substituting values of α = 0.57 and β = 0.43 in a theoretical model. These results demonstrate that the individual participation of the diploid genome A in the biosynthesis of the cytosolic aspartate aminotransferase allozymes subunits is greater than the individual participation of the diploid genomes B and D.

Highlights

Aspartate aminotransferase (AAT; EC 2.6.1.1) catalyses the fully reversible transamination reaction that occurs between L-aspartate and 2-oxoglutarate with the formation of oxaloacetate and L-glutamate
These results demonstrate that the individual participation of the diploid genome A in the biosynthesis of the cytosolic aspartate aminotransferase allozymes subunits is greater than the individual participation of the diploid genomes B and D
In order to specify chromosomal localization of the genes encoding a subunit of the cytosolic AAT allozymes, the following deletion lines of common wheat were used: del3AL-01, -03, -04, -05, -06, -08

Summary

Introduction

Aspartate aminotransferase (AAT; EC 2.6.1.1) catalyses the fully reversible transamination reaction that occurs between L-aspartate and 2-oxoglutarate with the formation of oxaloacetate and L-glutamate. Derivation of wheat deletion lines [15] allowed more precise localization of the genes encoding cytosolic allozymes on the chromosome Using such deletion lines Qi et al [16] established the localization of 7,104 expressed sequence tags (EST), including BF473016 EST which is a fragment of the sequence exhibiting a high degree of identity to the sequence of the genes encoding cytosolic AATs in different plants. It was mapped on the 3AL (0.4270.78), and on the short arms

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