Abstract
A tumour originating in the breast of a male rat was set up in culture and the cells cloned thirteen times. The chromosomes of the different clones differed slightly as to chromosomal mode, but they all contained two constant marker chromosomes. Ten of the thirteen clones were re-injected into cortisone prepared rats, but only five of these subsequently formed tumours in the animals. The chromosomes of the latter tumours were studied and they showed no significant shift in modal numbers or change in the number of marker chromosomes.
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